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PLEASE READ THE FULL GUIDE. Sterility and sterile technique is critical for mushroom growing. Familiarising yourself with these concepts ahead of time will massively increase your chances of a successful grow. Keep it clean folks!

For the inoculation phase, you want to be in a nice sanitary environment, or as clean as you can possibly find. A kitchen surface wiped down with 70% isopropyl alcohol will do the job. Make sure to eliminate as much draft as possible from your workspace.

Apply the gloves and face mask that are provided. Sanitise your hands and arms as well as the area that you are working in. 


1. Take your spore/culture syringe and shake it vigorously for 10 seconds. Remove the syringe cap by twisting. Fit the sterile needle onto the syringe, but DO NOT REMOVE THE COVER.

2. If you have been storing the bag in the fridge, let it sit at room temperature (>20°C) for 1 hour or so prior to inoculating. Make sure to mix the substrate gently in the bag prior to inoculation. This allows for an even distribution of moisture that the mycelium will love.

3. Wipe down the rubber injection port thoroughly with the alcohol swab provided. Allow a few seconds for the alcohol to evaporate before moving onto the next step.


4. Remove the cover from the syringe needle. Immediately after doing so, apply gentle pressure and pierce the sanitised injection port with the needle. Make sure to angle the needle towards the bottom of the bag, as shown above. This will help prevent the needle becoming blocked and allow you to see colonisation quicker.

Compress the syringe plunger and inject 3-6ml of solution. Remove the needle from the port. 

Congratulations! The inoculation procedure is complete and the bag is now ready for incubation.


Avoid getting any contaminants on the needle prior to injecting the kit. If you accidentally touch the needle at any point, you can flame sterilise it. Do so by applying a flame from a lighter to the tip of the needle until it glows red hot. Allow to cool and proceed with the steps.


1. Incubate the grain bag in a dark, warm spot. A cardboard box with some holes poked for air exchange works well. Keep the temperature between 21-24°C for optimal growth. Try not to exceed 26°C or you increase the risk of contamination, which is no bueno!

If you’re struggling to maintain a consistent temperature above 21°C, invest in a heat system such as our MushPro Incubator.

2. After about a week, you should start to see the first signs of colonisation. Healthy mycelium should appear first as fluffy white specs on the substrate. It can sometimes take longer than a week, so don’t panic. This is especially true if you’re using a spore syringe. Be prepared to wait! For quickest growth, use a liquid culture or agar.

3. Over the next 2-3 weeks, the mycelium will continue to grow and colonise the substrate. Once 30% of the substrate has been colonised, gently break apart the mycelium inside the bag and mix with the rest of the substrate. This will drastically speed up the colonisation process. 

4. Over the next week or so, the mycelium will consume all of the grain until it forms a white ‘cake’ of mycelium spawn. Once at this stage, you’re ready to spawn.


1. Once fully colonised, gently cut away the bag and remove the colonised cake. Carefully place the cake inside the large fruiting tent provided. Make sure to do this step in a clean area and always wash your hands thoroughly before handling the colonised substrate.

2. Give the inside walls of the bag a good misting with the mister bottle provided. Avoid misting the substrate directly.

Next, pin the bag shut with the paper clip. Place the fruiting tent in a warm spot between 20-22°c, where it can receive some indirect sunlight or room light for at least 4-6 hours a day. You do not need to overthink lighting as mushrooms require very little, but some is always beneficial.

3. For the first flush, very little misting is required. Usually a single misting on the inside walls of the fruiting tent is all that is needed. If the substrate is looking dry or there is little/no condensation on the inside of the fruiting tent, misting every couple of days may be required.

4. After about 14-21 days from spawning, you should see your first pins. These are baby mushrooms, that will grow and develop into mature mushroom fruiting bodies over the next few days.


Harvest the mushrooms just as the veils start to break. Once harvested, mist heavily directly onto the cake for 45 seconds to rehydrate it. Repeat fruiting steps 1-3 for subsequent flushes.

Expect 2 to 3 flushes (crops) of mushrooms per tub. Don’t throw the tub away after the first flush, there’s plenty more to come!

Your grow is finished once mushrooms no longer grow or the substrate eventually succumbs to contaminated. Dispose of the spent substrate in a compost heap.

As always, happy shrooming! 


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