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MonoTub Grow Guide

Stage 1: Inoculation

You want to be in a nice clean environment, or as clean as you can possibly find. A kitchen surface wiped down with isopropyl alcohol will do the job, make sure to eliminate as much draft as possible from your work space.

1

Put on the gloves and face mask which are supplied, sanitise your hands and arms as well as the area around you.

2

Take your spore/culture syringe and shake it vigorously to disperse the solution.

3

Quickly remove the protective cap from the syringe, and replace it with the sterile needle. Do not remove the needle cover just yet! 

4

Get your All-In-One bag and find the black injection port. Wipe it with the pre injection alcohol swab.

5

Take the syringe and remove the cap. Flame sterilise the needle with a lighter until the tip glows red hot. Give it a few seconds of time to cool before injecting.

6

Pierce the injection port that you have just wiped with alcohol (step 4). Inject around 3-4ml of solution into the millet grain.

Stage 2: Colonisation

1

Once inoculated, put your All-In-One bag somewhere dark that is away from direct sunlight, ambient light is fine. A cupboard works perfectly.

2

It is vital that you never open the bag up up before it is fully colonised and ready to fruit.

3

Keep your grow bag between 21-27°c for the fastest growth. Try not to exceed 27° or you risk the chance of contamination.

4

After about a week, you should start to see the first signs of colonisation. Healthy mycelium should appear first as fluffy white specs while it colonises the millet.

5

For the next 4-8 weeks your mycelium will colonise the millet in your All-In-One Bags. The bag will be ready to spawn into the MonoTub once the millet has all colonsed.

Stage 3: Spawning Your MonoTub

1

Firstly you will need to prepare your area, it is very important that your are as sterile as possible during this step. Poor sterile technique can lead to your MonoTub contaminating, essentially ruining your kit.

 

 

 

2

Put on the gloves and face mask which are supplied, sanitise your hands and arms as well as the area around you with the alcohol spray provided.

3

Using the alcohol spray, sanitise the entire MonoTub. Close the lid on the tub ensuring the inside is kept sterile.

4

Now spray your All-In-One bag with alcohol to sanitise the outside of the bag. Break the colonised millet up and mix it with the bulk substrate so that it is evenly mixed up.

5

Cut the All-In-One bag open and empty it inside the MonoTub, even out the substrate with your hands to create a flat surface. Put the lid on your MonoTub immediately after and make sure it is firmly closed.

6

Put your MonoTub somewhere dark that is away from direct sunlight, ambient light is fine. A cupboard works perfectly. You want temperatures of 20-25°

7

After about 10 days your MonoTub should be fully colonised and ready to fruit, don’t worry if it hasn’t fully colonised by then. It can sometimes take as long as 2/3 weeks to fully colonise.

Stage 4: Fruiting

1

To put your MonoTub into fruiting, just flip the lid! This creates a small gap that allows for air exchange.

2

Place your MonoTub in a warm spot between 20-24°c, where they can receive some indirect sunlight. You want to give the substrate at least a few hours of sunlight a day.

3

You want to lightly mist your MonoTub once a day with the mister bottle provided. After misting you want to lightly fan with lid of from the MonoTub.

4

After about 10-14 days should see your first pins, which are the first tiny mushroom fruits.

Stage 5: Harvesting

1

Once the veils break and your mushroom caps start to flatten out, they are ready for harvest. You can either use scissors or a scalpel to cut the mushrooms off the cake.

2

After harvesting the first flush give the substrate block a good misting, and repeat Fruiting steps 1-4.

3

Keep repeating until the substrate no longer produces fruits, or until it gets contaminated.

Optimising Your Grow: Tips and Tricks

  • Always try to be as sterile as possible, you can never take sterility too far. You don’t want to put all this time, effort and money in only to get a contamination that ruins your grow.                                                                                                                                                                                 
  • If you are using a heating matt, be aware that it can cause stalling. Because the uneven heating of the matt, it warms up and dries out the bottom layer of your substrate. This is what can cause it to stall. So you might want to turn your heating matt off every now and then to try to prevent this. If you are using one of our incubators then you will be fine to keep it at optimal temperatures at all times, as the incubator heats the entire area evenly, which maintains a balanced and stable heating environment. Just make sure the temperatures do not exceed 27° when using our incubators, as they can get very warm.

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